Molecular and serological studies of FMD virus serotype (O)

Virology Department, Animal Health Research Institute, P.O.Box264, Dokki, 12618, Giza, Egypt

Abstract
Foot-and-mouth disease (FMD) is a severe, highly contagious and economically devastating viral disease worldwide, which affects animals with cloven hooves including domestic and wild bovids.
In Egypt three strains circulating in ruminant livestock in different governorates causing outbreaks in both vaccinated and non-vaccinated animals.
In the current study fifty Samples showing clinical signs similar to FMD were collected from different governorates in Egypt during winter 2017; This was aimed to quantify the extent of genetic diversity of FMDV in Egypt by sequencing.
Samples were subjected to antigenic diagnosis by antigen detection ELISA and molecular diagnosis by PCR followed by partial sequence analysis of VP1 gene of the PCR product.
Multiple alignment and phylogenetic analysis of VP1 were done indicating the presence of FMD V – type O topotype East Africa3. Identity percent between field strains and Ismailia Egypt 2016 was 96.2% and with Egypt 2014, 94.7% and similarity with O Sudan –EA3 is 96.2% ; in case of O Sharqia 2014 the homology percent reached to 93.1%